How to get into flow with it: measuring the paradoxes in digital knowledge work

Digitized knowledge workers are exposed to various technology-, individual- and work-related factors resulting in multiple paradoxes that may promote or hinder their capacity to work. This paper elaborates on how emerging paradoxes of IT usage impact the flow experience for daily planning tasks of knowledge workers. To study the impact beyond effective use of IT on flow, we conducted a survey study with 336 participants in a mixed-method approach combining PLS-SEM and fsQCA. Our results show that the digital working method could positively influence the flow experience overall. A full mediation of perceived behavioral control, representing the paradox control and chaos, and representational fidelity, representing clarity and ambiguity, on flow, was confirmed. Our fsQCA results support the conclusion that increasing IT penetration alone is insufficient to experience work flow. It depends on how knowledge workers interact with the IT in their specific task environment, balancing the dialectical tensions at work, with some differences between genders and within specific industries. We discuss the study\u27s implications for research and practice

UNSTEADY BOUNDARY LAYER TRANSITION ON A 11111111111lligium HIGH PRESSURE TURBINE ROTOR BLADE

99-GT-194 The Society shall not be responsible for statements or opinions advanced in papers or discussion at meetings of the Society or of its Divisions or Sections, or printed in its publications. Discussioeis printed only if the paper is published in an AS ME Journal. ABSTRACT This investigation is aimed at the experimental determination of the location, the extent, and the modes of the laminar-to-turbulent transition processes in the boundary layers of a high pressure turbine rotor blade. The results are based on time-resolved, qualitative wall shear stress data which was derived from surface hotfilm measurements. The tests were conducted in the "Windtunnel for Rotating Cascades" of the DLR in Gottingen. For the evaluation of the influence of passing wakes and shocks on the unsteady boundary layer transition, a test with undisturbed rotor inlet flow was conducted in addition to full stage tests. Two different transition modes led to a periodic-unsteady, multimoded transition on the suction side. In between two wakes, transition started in the bypass mode and terminated as separated-flow transition. Underneath the wakes, plain bypass transition occurred. The weak periodic boundary layer features on the pressure side indicate that this surface was not significantly affected by passing wakes or shocks. The acquired data reveals that the periodically disturbed suction side boundary layer is less susceptible to bubble bursting than the undisturbed flowfield. Thus, these blades may be subjected to higher aerodynamic loads. Accordingly, as in low pressure turbines, the unsteady effects in high pressure turbines may allow for a reduction of the number of rotor blades, with respect to the original design

The cDNA sequences of cytochrome c oxidase subunit VIa from carp and rainbow trout suggest the absence of isoforms in fishes1The sequence data in this paper have been submitted to the GenBank data library under the accession numbers: BankIt88656 U83980 (trout) and BankIt88644 U83907 (carp).1

AbstractThe cDNAs of subunit VIa of cytochrome c oxidase from rainbow trout liver and carp heart are presented, revealing 82% identity of their deduced amino acid sequences. The two cDNAs are evolutionary equally distant from the livertype (VIaL) and heart-type (VIaH) of mammalian subunit VIa. The data suggest that in ectotherm fishes no isoforms of subunit VIa occur, and that the postulated tissue-specific mechanism of thermogenesis in mammals, based on interaction of ATP with subunit VIaH (Frank, V. and Kadenbach, B. (1996) FEBS Lett. 382, 121–124), is absent.© 1997 Elsevier Science B.V. All rights reserved

Identification of two regulatory binding sites which confer myotube specific expression of the mono-ADP-ribosyltransferase ART1 gene

<p>Abstract</p> <p>Background</p> <p>Mono-ADP-ribosyltransferase (ART) 1 belongs to a family of mammalian ectoenzymes that catalyze the transfer of ADP-ribose from NAD⁺to a target protein. ART1 is predominantly expressed in skeletal and cardiac muscle. It ADP-ribosylates α7-integrin which together with β1-integrin forms a dimer and binds to laminin, a protein of the extracellular matrix involved in cell adhesion. This posttranslational modification leads to an increased laminin binding affinity.</p> <p>Results</p> <p>Using C2C12 and C3H-10T 1/2 cells as models of myogenesis, we found that ART1 expression was restricted to myotube formation. We identified a fragment spanning the gene 1.3 kb upstream of the transcriptional start site as the functional promoter of the <it>ART1 </it>gene. This region contains an E box and an A/T-rich element, two conserved binding sites for transcription factors found in the promoters of most skeletal muscle specific genes. Mutating the DNA consensus sequence of either the E box or the A/T-rich element resulted in a nearly complete loss of <it>ART1 </it>promoter inducibility, indicating a cooperative role of the transcription factors binding to those sites. Gel mobility shift analyses carried out with nuclear extracts from C2C12 and C3H-10T 1/2 cells revealed binding of myogenin to the E box and MEF-2 to the A/T-rich element, the binding being restricted to C2C12 and C3H-10T 1/2 myotubes.</p> <p>Conclusion</p> <p>Here we describe the molecular mechanism underlying the regulation of the <it>ART1 </it>gene expression in skeletal muscle cells. The differentiation-dependent upregulation of ART1 mRNA is induced by the binding of myogenin to an E box and of MEF-2 to an A/T-rich element in the proximal promoter region of the <it>ART1 </it>gene. Thus the transcriptional regulation involves molecular mechanisms similar to those used to activate muscle-specific genes.</p

Effects of hemodialysis on plasma oxylipins

Chronic kidney disease (CKD) is an important risk factor for cardiovascular and all-cause mortality. Survival rates among end-stage renal disease (ESRD) hemodialysis patients are poor and most deaths are related to cardiovascular disease. Oxylipins constitute a family of oxygenated natural products, formed from fatty acid by pathways involving at least one step of dioxygen-dependent oxidation. They are derived from polyunsaturated fatty acids (PUFAs) by cyclooxygenase (COX) enzymes, by lipoxygenases (LOX) enzymes, or by cytochrome P450 epoxygenase. Oxylipins have physiological significance and some could be of regulatory importance. The effects of decreased renal function and dialysis treatment on oxylipin metabolism are unknown. We studied 15 healthy persons and 15 CKD patients undergoing regular hemodialysis treatments and measured oxylipins (HPLC-MS lipidomics) derived from cytochrome P450 (CYP) monooxygenase and lipoxygenase (LOX)/CYP omega/(omega-1)-hydroxylase pathways in circulating blood. We found that all four subclasses of CYP epoxy metabolites were increased after the dialysis treatment. Rather than resulting from altered soluble epoxide hydrolase (sEH) activity, the oxylipins were released and accumulated in the circulation. Furthermore, hemodialysis did not change the majority of LOX/CYP omega/(omega-1)-hydroxylase metabolites. Our data support the idea that oxylipin profiles discriminate ESRD patients from normal controls and are influenced by renal replacement therapies

The premenstrual syndrome and self-report

This study reviews the literature on the premenstrual syndrome specifically prevalence, definitional issues, etiological assumptions, and methodological issues. The conclusion is reached that the area requires the input of further well-controlled research. The technique of self-report is also reviewed with particular emphasis on applications, methodological advantages and limitations. The particular relevance of self-report to the study of the premenstrual syndrome is addressed as is the validity of self-report in the area. The first aim was to collect sufficient daily mood data from target subjects to determine correlations with their menstrual cycle. Secondly, data was collected from significant other observers and cyclicity was measured. Lastly, the relationship between target-rated and observer-rated mood was analysed for commonly occurring cyclicity. The statistical technique involved was Spectral Analysis. Thirteen subjects and observers were involved and results showed clear evidence of PMS in three subjects confirmed by observer recordings. Lack of coherence in cyclicity between target mood and observer rated mood was shown. It was concluded that 1) some of these women may not have been suffering from PMS and that 2) lack of coherence between target and observer on mood ratings was due to the 'private' nature of mood or to poor observation

Urine Metabolomics by 1H-NMR Spectroscopy Indicates Associations between Serum 3,5-T2 Concentrations and Intermediary Metabolism in Euthyroid Humans

Context: 3,5-Diiodo-L-thyronine (3,5-T2) is a thyroid hormone metabolite which exhibited versatile effects in rodent models, including the prevention of insulin resistance or hepatic steatosis typically forced by a high-fat diet. With respect to euthyroid humans, we recently observed a putative link between serum 3,5-T2 and glucose but not lipid metabolism. Objective: The aim of the present study was to widely screen the urine metabolome for associations with serum 3,5-T2 concentrations in healthy individuals. Study Design and Methods: Urine metabolites of 715 euthyroid participants of the population-based Study of Health in Pomerania (SHIP-TREND) were analyzed by 1H-NMR spectroscopy. Multinomial logistic and multivariate linear regression models were used to detect associations between urine metabolites and serum 3,5-T2 concentrations. Results: Serum 3,5-T2 concentrations were positively associated with urinary levels of trigonelline, pyroglutamate, acetone and hippurate. In detail, the odds for intermediate or suppressed serum 3,5-T2 concentrations doubled owing to a 1-standard deviation (SD) decrease in urine trigonelline levels, or increased by 29-50% in relation to a 1-SD decrease in urine pyroglutamate, acetone and hippurate levels. Conclusion: Our findings in humans confirmed the metabolic effects of circulating 3,5-T2 on glucose and lipid metabolism, oxidative stress and enhanced drug metabolism as postulated before based on interventional pharmacological studies in rodents. Of note, 3,5-T2 exhibited a unique urinary metabolic profile distinct from previously published results for the classical thyroid hormones

Comprehensive metabolic profiling of chronic low-grade inflammation among generally healthy individuals.

BACKGROUND: Inflammation occurs as an immediate protective response of the immune system to a harmful stimulus, whether locally confined or systemic. In contrast, a persisting, i.e., chronic, inflammatory state, even at a low-grade, is a well-known risk factor in the development of common diseases like diabetes or atherosclerosis. In clinical practice, laboratory markers like high-sensitivity C-reactive protein (hsCRP), white blood cell count (WBC), and fibrinogen, are used to reveal inflammatory processes. In order to gain a deeper insight regarding inflammation-related changes in metabolism, the present study assessed the metabolic patterns associated with alterations in inflammatory markers. METHODS: Based on mass spectrometry and nuclear magnetic resonance spectroscopy we determined a comprehensive panel of 613 plasma and 587 urine metabolites among 925 apparently healthy individuals. Associations between inflammatory markers, namely hsCRP, WBC, and fibrinogen, and metabolite levels were tested by linear regression analyses controlling for common confounders. Additionally, we tested for a discriminative signature of an advanced inflammatory state using random forest analysis. RESULTS: HsCRP, WBC, and fibrinogen were significantly associated with 71, 20, and 19 plasma and 22, 3, and 16 urine metabolites, respectively. Identified metabolites were related to the bradykinin system, involved in oxidative stress (e.g., glutamine or pipecolate) or linked to the urea cycle (e.g., ornithine or citrulline). In particular, urine 3'-sialyllactose was found as a novel metabolite related to inflammation. Prediction of an advanced inflammatory state based solely on 10 metabolites was well feasible (median AUC: 0.83). CONCLUSIONS: Comprehensive metabolic profiling confirmed the far-reaching impact of inflammatory processes on human metabolism. The identified metabolites included not only those already described as immune-modulatory but also completely novel patterns. Moreover, the observed alterations provide molecular links to inflammation-associated diseases like diabetes or cardiovascular disorders

Aging Affects KV7 Channels and Perivascular Adipose Tissue-Mediated Vascular Tone

Aging is an independent risk factor for hypertension, cardiovascular morbidity, and mortality. However, detailed mechanisms linking aging to cardiovascular disease are unclear. We studied the aging effects on the role of perivascular adipose tissue and downstream vasoconstriction targets, voltage-dependent KV7 channels, and their pharmacological modulators (flupirtine, retigabine, QO58, and QO58-lysine) in a murine model. We assessed vascular function of young and old mesenteric arteries in vitro using wire myography and membrane potential measurements with sharp electrodes. We also performed bulk RNA sequencing and quantitative reverse transcription-polymerase chain reaction tests in mesenteric arteries and perivascular adipose tissue to elucidate molecular underpinnings of age-related phenotypes. Results revealed impaired perivascular adipose tissue-mediated control of vascular tone particularly via KV7.3–5 channels with increased age through metabolic and inflammatory processes and release of perivascular adipose tissue-derived relaxation factors. Moreover, QO58 was identified as novel pharmacological vasodilator to activate XE991-sensitive KCNQ channels in old mesenteric arteries. Our data suggest that targeting inflammation and metabolism in perivascular adipose tissue could represent novel approaches to restore vascular function during aging. Furthermore, KV7.3–5 channels represent a promising target in cardiovascular aging

The influence of training level on manual flight in connection to performance, scan pattern, and task load.

This work focuses on the analysis of pilots’ performance during manual flight operations in different stages of training and situations and also examines the influence of training on gaze strategy. The secure and safe operation of air traffic is highly dependent on the individual performances of the pilots. Before becoming a pilot, he/she has to acquire a broad set of skills by training to pass all the necessary qualification and licensing standards. A basic skill for every pilot is manual control operations, which is a closed-loop control process with several cross-coupled variables. Even with increased automation in the cockpit, the manual control operations are essential for every pilot as a last resort in the event of automation failure. A key element in the analysis of manual flight operations is the development over time in relation to performance and visual perception. An experiment with 28 participants (including 11 certified pilots) was conducted in a Boeing 737 simulated in a high-fidelity setting. For defined flight phases, the dynamic time warping method was applied to evaluate the performance for selected criteria, and eye-tracking methodology was utilized to analyze the gaze-pattern development. The manipulation of experience and workload influences the performance and the gaze pattern at the same time. Findings suggest that the increase of workload has an increased effect on pilots depending on the flight phase. Gaze patterns from experienced pilots provide insights into the training requirements of both novices and experts. The connection between workload, performance and gaze pattern is complex and needs to be analyzed under as many differing conditions. The results imply the necessity to evaluate manual flight operations with respect to more flight phases and a detailed selection of performance indications